PA5-46993

antibody from Invitrogen Antibodies

Targeting: IL27 IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46993

Provider

Invitrogen Antibodies

Product name

IL-27 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse IL-27, recombinant human (rh) IL-12, and rhIL-12 p40 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL-27 is shown by the decrease in mean RFU (relative fluorescent units - measure of inhibition of EMCV-induced cytopathy) with increasing concentrations of IL15RA Polyclonal Antibody (PA5-46993).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of IL-27 in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in IL-27 polyclonal antibody (Product # PA5-46993). Recombinant Human IL‚27 reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL‚27 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚27 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1‚5 µg/mL.