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antibody from Invitrogen Antibodies
Targeting: IL27
IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28
Western blot
ELISA
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-46993 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse IL-27, recombinant human (rh) IL-12, and rhIL-12 p40 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-27 in MT‚2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. Samples were incubated in IL-27 polyclonal antibody (Product # PA5-46993) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for the IL‚27 EBI3/IL‚27B at approximately 32 kDa and IL‚27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL-27 in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in IL-27 polyclonal antibody (Product # PA5-46993). Recombinant Human IL‚27 reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL‚27 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚27 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1‚5 µg/mL.
