PA5-63890

antibody from Invitrogen Antibodies

Targeting: EHF ESE3, ESEJ

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-63890

Provider

Invitrogen Antibodies

Product name

EHF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MILEGGGVMN LNPGNNLLHQ PPAWTDSYST CNVSSGFFGG QWHEIHPQYW TKYQVWEWLQ HLLDTNQLDA NCIPFQEFDI NGEHL Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 94%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma.

Zhang T, Song X, Zhang Z, Mao Q, Xia W, Xu L, Jiang F, Dong G
Oncogenesis 2020 Oct 17;9(10):92

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 High expression of CRC (ELF3/EHF/TGIF1-High) were associated with poor prognosis on patients with LUAD. A qRT-PCR analysis of ELF3, EHF, and TGIF1 among 20 pairs of matched lung adenocarcinoma and adjacent normal tissue. B Correlation analyses conducted between the mRNA expression of ELF3, EHF, and TGIF1 from ( A ). C ELF3, EHF, and TGIF1 were detected in peritumoral tissue and representative LUAD samples by immunohistochemistry staining. Scale bar, 100 mum. D Kaplan-Meier analysis of overall survival of LUAD patients (TCGA-LUAD) with low (low expression of ELF3, EHF, and TGIF1, n = 78) and high (high expression of ELF3, EHF, and TGIF1, n = 52) ELF3/EHF/TGIF1 expression (log-rank test, two-sided). E Left panel: a schematic diagram showing the key targets and small-molecule inhibitors of SE. JQ1 and OTX015 targeting BRD4, THZ1 targeting CDK7; CBPC30 targeting EP300. Right panel: CRCs are assembled as fully interconnected loops of auto-regulated TFs. Schematic graph of the model of interconnected circuitry, with hexagons representing enhancer elements and proteins, respectively. Data in ( A ) were mean +- SD; n = 3 independent experiments, two-tailed paired Student's t test, ns not significant, * p < 0.05; ** p < 0.01; *** p < 0.001 ( B ) R, Pearson correlation coefficients ( r ) and p values.