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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-30716 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-EHF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T. Predicted reactivity: Mouse (92%), Rat (92%), Chicken (80%), Chimpanzee (99%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.88 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma.
Liu J, Jiang W, Zhao K, Wang H, Zhou T, Bai W, Wang X, Zhao T, Huang C, Gao S, Qin T, Yu W, Yang B, Li X, Fu D, Tan W, Yang S, Ren H, Hao J
The Journal of experimental medicine 2019 Mar 4;216(3):656-673
The Journal of experimental medicine 2019 Mar 4;216(3):656-673
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EHF using 30 µg of 293T lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an EHF polyclonal antibody (Product # PA5-30716) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EHF in paraformaldehyde-fixed HeLa cells using an EHF polyclonal antibody (Product # PA5-30716) at a 1:500 dilution.
