PA5-82152

antibody from Invitrogen Antibodies

Targeting: TRIM33 FLJ11429, KIAA1113, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-82152

Provider

Invitrogen Antibodies

Product name

TRIM33 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MQPHLQRQHS NPGHAGPFPV VSVHNTTINP TSPTTATMAN ANRGPTSPSV TAIELIPSVT NPENLPSLPD IPPIQLEDAG SSSLDNLLSR YISGSHLPPQ PTSTMNPSPG PSALSPGSSG LSNSHTPVRP PSTSSTGSR

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Identification of SUMO Targets Associated With the Pluripotent State in Human Stem Cells.

Mojsa B, Tatham MH, Davidson L, Liczmanska M, Branigan E, Hay RT
Molecular & cellular proteomics : MCP 2021;20:100164

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TRIM33 in U-251 MG cells using a TRIM33 polyclonal antibody (Product # PA5-82152). The analysis shows localization to nucleoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 Functions of SUMO1 and SUMO2 targets in hiPSCs. A , in total, 976 SUMO sites were identified from 6His-SUMO1-KGG and 6His-SUMO2-KGG IPS cells, of which 155 were novel compared with previous high-throughput SUMO site proteomics studies ( supplemental File S2 ). Proteins with three or more novel sites are highlighted. B , summary of the top 50 SUMO substrates by total SUMO1+SUMO2 GGK-peptide intensity for all identified sites. Gene names are shown with numbers of sites in brackets . Bars are color-coded by category shown in panel D . Insert shows contribution to total GGK peptide intensity of proteins from the categories shown in ( D ) (note categories are not mutually exclusive). C , immunoblot analysis of ChIPS4 cells treated with the SUMO E1 conjugating enzyme inhibitor ML792 or DMSO for 48 h. Total protein extracts were probed with anti-TRIM28, anti-TRIM24, anti-CTCF, anti-DNMT3b, anti-SALL4, anti-TRIM33, and anti-tubulin or anti-Lamin A/C antibodies (loading controls). SUMO-modified species above the band for unmodified proteins (*) reduce with ML792 treatment. D , STRING interaction network of the 427 IPS SUMO substrates. Only high confidence interactions were considered from ""Text mining,"" ""Experiments,"" and ""Databases"" sources. Network PPI enrichment p -value