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LearnA301-059A
antibody from Invitrogen Antibodies
Targeting: TRIM33
FLJ11429, KIAA1113, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA
Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A301-059A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM33/TIF1gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse TRIM33/TIF1gamma by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- TRIM33/TIF1gamma (Cat. No. A301-059A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human T RIM33/TIF1gamma by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti- TRIM33/TIF1gamma (Cat. No. A301-059A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TRIM33/TIF1gamma by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIM33/TIF1gamma antibody A301-059A (lot A301-059A-2) used for IP at 6 µg per reaction. TRIM33/TIF1gamma was also immunoprecipitated by a previous lot of this antibody (Lot A301-059A-1) and rabbit anti-TRIM33/TIF1gamma antibody A301-060A. For blotting immunoprecipitated TRIM33/TIF1gamma, A301-059A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
