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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-29456 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, U87-MG.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RNF14 Polyclonal Antibody (Product # PA5-29456). Sample (30 µg of whole cell lysate). Lane A: IMR32. 10% SDS PAGE. RNF14 Polyclonal Antibody (Product # PA5-29456) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNF14 Polyclonal Antibody detects RNF14 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RNF14 protein stained by RNF14 Polyclonal Antibody (Product # PA5-29456) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
