Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [16]
Submit
Validation data
Reference
Comment
Report error
- Product number
- OSC00009W - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cathelicidin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAP-18 using a polyclonal antibody (Product # OSC00009W).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on different lysates using Cathelicidin Polyclonal Antibody (Product #OSC00009W). Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1,000 incubated at 4C overnight.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CAP-18 using a polyclonal antibody (Product # OSC00009W).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0. 1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Cathelicidin Polyclonal Antibody (Product #OSC00009W) diluted 1:200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis performed on ethanol-fixed human spermatosoid using Cathelicidin Polyclonal Antibody (Product # OSC00009W) at 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human appendix tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human prostate tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human skin tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human tonsil tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human tonsil tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human tonsil tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human tonsil tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human thyroid tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human fallopian tube tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human kidney tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human lung tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human large intestine tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human large intestine tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human liver tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human liver tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed on sections of human placenta tissue using Cathelicidin Polyclonal Antibody (Product # OSC00009W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
