PA5-110314

antibody from Invitrogen Antibodies

Targeting: RPA3 REPA3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-110314

Provider

Invitrogen Antibodies

Product name

RPA14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DLPRSRINAG MLAQFIDKPV CFVGRLEKIH PTGKMFILSD GEGKNGTIEL MEPLDEEISG IVEVVGRVTA KATILCTSYV QFKEDSHPFD LGLYNEAVKI IHDFPQFYPL GIVQHD

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.67 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPA14 in extracts of various cell lines. Samples were incubated with RPA14 Polyclonal antibody (Product # PA5-110314) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of RPA14 in MCF7. Samples were incubated in polyclonal RPA14 antibody (Product # PA5-110314) using a dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPA14 in MCF7 cells. Samples were incubated with RPA14 Polyclonal antibody (Product # PA5-110314).