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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- NBP2-22591 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PIG3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PIG3 Antibody (1C9) [NBP2-22591] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PIG3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : SW480 cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PIG3 Antibody (1C9) [NBP2-22591] - The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PIG3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PIG3 Transfected 293T cell lysate
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PIG3 Antibody (1C9) [NBP2-22591] - Flow cytometry analysis of PIG3 in A549 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
