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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-52032 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VKSGVFNVSE LVRVSRTPIT QGTGVNFPIG EIPSQPYYHD MNSGVNLQRS LSSPPSSKRP KTISIDENME PSPTGDFYPS PSSPAAGSRT WHERDQDMSS PTTMKKPEKP LFSSASPQDS SPRLSTFPQH HHPGIPGVAH SVISTRTP Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.15 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A positive feedback loop involving nuclear factor IB and calpain 1 suppresses glioblastoma cell migration.
Vo TM, Jain S, Burchett R, Monckton EA, Godbout R
The Journal of biological chemistry 2019 Aug 23;294(34):12638-12654
The Journal of biological chemistry 2019 Aug 23;294(34):12638-12654
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIB in human cell line SH-SY5Y using a NFIB Polyclonal Antibody (Product # PA5-52032).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIB in mouse cell line NIH-3T3 and rat cell line NBT-II using a NFIB Polyclonal Antibody (Product # PA5-52032).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NFIB in human cell line U-251 MG shows positivity in nucleoli & nucleus but excluded from the nucleoli. Samples were probed using a NFIB Polyclonal Antibody (Product # PA5-52032).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NFIB in human cell line U-251 MG using a NFIB Polyclonal Antibody (Product # PA5-52032) shows localization to nucleoplasm and nucleoli fibrillar center.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIB in human heart muscle shows strong nuclear positivity in myocytes. Samples were probed using a NFIB Polyclonal Antibody (Product # PA5-52032).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
