PA5-52032

antibody from Invitrogen Antibodies

Targeting: NFIB NFI-RED, NFIB2, NFIB3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52032

Provider

Invitrogen Antibodies

Product name

NFIB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VKSGVFNVSE LVRVSRTPIT QGTGVNFPIG EIPSQPYYHD MNSGVNLQRS LSSPPSSKRP KTISIDENME PSPTGDFYPS PSSPAAGSRT WHERDQDMSS PTTMKKPEKP LFSSASPQDS SPRLSTFPQH HHPGIPGVAH SVISTRTP Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.15 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A positive feedback loop involving nuclear factor IB and calpain 1 suppresses glioblastoma cell migration.

Vo TM, Jain S, Burchett R, Monckton EA, Godbout R
The Journal of biological chemistry 2019 Aug 23;294(34):12638-12654

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NFIB in human cell line U-251 MG shows positivity in nucleoli & nucleus but excluded from the nucleoli. Samples were probed using a NFIB Polyclonal Antibody (Product # PA5-52032).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NFIB in human cell line U-251 MG using a NFIB Polyclonal Antibody (Product # PA5-52032) shows localization to nucleoplasm and nucleoli fibrillar center.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL