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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-34902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIB Monoclonal Antibody (1H2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SH-SY-5Y, LO-2, rat heart tissue, human colon cancer tissue, human prostate tissue, human pancreas tissue, mouse liver tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1H2
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NFIB NF1B2 in LO-2 cells. Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of NFIB NF1B2 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with NFIB NF1B2 monoclonal antibody (Product # MA5-34902) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG.
