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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [3]
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- Product number
- RQ6747 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nuclear factor 1 B-type Antibody / NFIB
- Antibody type
- Monoclonal
- Description
- This highly specific Nuclear factor 1 B-type antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4D6E4
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Nuclear factor 1 B-type antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) 293T cell lysate with Nuclear factor 1 B-type antibody. Predicted molecular weight ~47 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with Nuclear factor 1 B-type antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with Nuclear factor 1 B-type antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nuclear factor 1 B-type antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse Neuro-2a cells with Nuclear factor 1 B-type antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nuclear factor 1 B-type antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of rat C6 cells with Nuclear factor 1 B-type antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nuclear factor 1 B-type antibody.
