HPA003956

antibody from Atlas Antibodies

Targeting: NFIB NFI-RED, NFIB2, NFIB3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA003956

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003956, RRID:AB_1854424

Product name

Anti-NFIB

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human NFIB, Gene description: nuclear factor I/B, Alternative Gene Names: NFI-RED, NFIB2, NFIB3, Validated applications: ChIP, ICC, WB, Uniprot ID: O00712, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

TRIM71 reactivation enhances the mitotic and hair cell–forming potential of cochlear supporting cells

Li X, Morgan C, Nadar‐Ponniah P, Kolanus W, Doetzlhofer A
EMBO reports 2023;24(9)

---

Cell-specific and shared regulatory elements control a multigene locus active in mammary and salivary glands

Lee H, Willi M, Liu C, Hennighausen L
Nature Communications 2023;14(1)

---

PTHrP induces STAT5 activation, secretory differentiation and accelerates mammary tumor development

Grinman D, Boras-Granic K, Takyar F, Dann P, Hens J, Marmol C, Lee J, Choi J, Chodosh L, Sola M, Wysolmerski J
Breast Cancer Research 2022;24(1)

---

The Polymorphic Nuclear Factor NFIB Regulates Hepatic CYP2D6 Expression and Influences Risperidone Metabolism in Psychiatric Patients

Lenk H, Klöditz K, Johansson I, Smith R, Jukić M, Molden E, Ingelman‐Sundberg M
Clinical Pharmacology & Therapeutics 2022;111(5):1165-1174

---

ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer

Olsen R, Ireland A, Kastner D, Groves S, Spainhower K, Pozo K, Kelenis D, Whitney C, Guthrie M, Wait S, Soltero D, Witt B, Quaranta V, Johnson J, Oliver T
Genes & Development 2021;35(11-12):847-869

---

The NFIB‐ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells

Zilli F, Marques Ramos P, Auf der Maur P, Jehanno C, Sethi A, Coissieux M, Eichlisberger T, Sauteur L, Rouchon A, Bonapace L, Pinto Couto J, Rad R, Jensen M, Banfi A, Stadler M, Bentires‐Alj M
EMBO Molecular Medicine 2021;13(4)

---

Redundant and non-redundant cytokine-activated enhancers control Csn1s2b expression in the lactating mouse mammary gland

Lee H, Willi M, Kuhns T, Liu C, Hennighausen L
Nature Communications 2021;12(1)

---

Rlf–Mycl Gene Fusion Drives Tumorigenesis and Metastasis in a Mouse Model of Small Cell Lung Cancer

Ciampricotti M, Karakousi T, Richards A, Quintanal-Villalonga À, Karatza A, Caeser R, Costa E, Allaj V, Manoj P, Spainhower K, Kombak F, Sanchez-Rivera F, Jaspers J, Zavitsanou A, Maddalo D, Ventura A, Rideout W, Akama-Garren E, Jacks T, Donoghue M, Sen T, Oliver T, Poirier J, Papagiannakopoulos T, Rudin C
Cancer Discovery 2021;11(12):3214-3229

---

DCC regulates astroglial development essential for telencephalic morphogenesis and corpus callosum formation

Morcom L, Gobius I, Marsh A, Suárez R, Lim J, Bridges C, Ye Y, Fenlon L, Zagar Y, Douglass A, Donahoo A, Fothergill T, Shaikh S, Kozulin P, Edwards T, Cooper H, Sherr E, Chédotal A, Leventer R, Lockhart P, Richards L
eLife 2021;10

---

Increased nuclear factor I/B expression in prostate cancer correlates with AR expression

Nanda J, Awadallah W, Kohrt S, Popovics P, Cates J, Mirosevich J, Clark P, Giannico G, Grabowska M
The Prostate 2020;80(13):1058-1070

---

Regionally Distinct Astrocytes Display Unique Transcription Factor Profiles in the Adult Brain

Lozzi B, Huang T, Sardar D, Huang A, Deneen B
Frontiers in Neuroscience 2020;14

---

Region-Specific Transcriptional Control of Astrocyte Function Oversees Local Circuit Activities

Huang A, Woo J, Sardar D, Lozzi B, Bosquez Huerta N, Lin C, Felice D, Jain A, Paulucci-Holthauzen A, Deneen B
Neuron 2020;106(6):992-1008.e9

---

Chromatin Decondensation by FOXP2 Promotes Human Neuron Maturation and Expression of Neurodevelopmental Disease Genes

Hickey S, Berto S, Konopka G
Cell Reports 2019;27(6):1699-1711.e9

---

YAP1 subgroup supratentorial ependymoma requires TEAD and nuclear factor I-mediated transcriptional programmes for tumorigenesis.

Pajtler KW, Wei Y, Okonechnikov K, Silva PBG, Vouri M, Zhang L, Brabetz S, Sieber L, Gulley M, Mauermann M, Wedig T, Mack N, Imamura Kawasawa Y, Sharma T, Zuckermann M, Andreiuolo F, Holland E, Maass K, Körkel-Qu H, Liu HK, Sahm F, Capper D, Bunt J, Richards LJ, Jones DTW, Korshunov A, Chavez L, Lichter P, Hoshino M, Pfister SM, Kool M, Li W, Kawauchi D
Nature communications 2019 Sep 2;10(1):3914

---

NFIB Mediates BRN2 Driven Melanoma Cell Migration and Invasion Through Regulation of EZH2 and MITF

Fane M, Chhabra Y, Hollingsworth D, Simmons J, Spoerri L, Oh T, Chauhan J, Chin T, Harris L, Harvey T, Muscat G, Goding C, Sturm R, Haass N, Boyle G, Piper M, Smith A
EBioMedicine 2017;16

---

Combined allelic dosage of Nfia and Nfib regulates cortical development

Bunt J, Osinski J, Lim J, Vidovic D, Ye Y, Zalucki O, O’Connor T, Harris L, Gronostajski R, Richards L, Piper M
Brain and Neuroscience Advances 2017;1

---

Nfib Regulates Transcriptional Networks That Control the Development of Prostatic Hyperplasia

Matusik R, Anderson P, Gronostajski R, Hayward S, Clark P, Miller N, Strand D, DeGraff D, Zhang J, Case T, Cates J, Reese A, Kelly S, Grabowska M
Endocrinology 2016;157(3):1094-1109

---

A Genome-Wide Scan Identifies Variants in NFIB Associated with Metastasis in Patients with Osteosarcoma

Mirabello L, Koster R, Moriarity B, Spector L, Meltzer P, Gary J, Machiela M, Pankratz N, Panagiotou O, Largaespada D, Wang Z, Gastier-Foster J, Gorlick R, Khanna C, de Toledo S, Petrilli A, Patiño-Garcia A, Sierrasesúmaga L, Lecanda F, Andrulis I, Wunder J, Gokgoz N, Serra M, Hattinger C, Picci P, Scotlandi K, Flanagan A, Tirabosco R, Amary M, Halai D, Ballinger M, Thomas D, Davis S, Barkauskas D, Marina N, Helman L, Otto G, Becklin K, Wolf N, Weg M, Tucker M, Wacholder S, Fraumeni J, Caporaso N, Boland J, Hicks B, Vogt A, Burdett L, Yeager M, Hoover R, Chanock S, Savage S
Cancer Discovery 2015;5(9):920-931

---

PAX6 does not regulate Nfia and Nfib expression during neocortical development

Bunt J, Lim J, Zhao L, Mason S, Richards L
Scientific Reports 2015;5(1)

---

NFIB-Mediated Repression of the Epigenetic FactorEzh2Regulates Cortical Development

Piper M, Barry G, Harvey T, McLeay R, Smith A, Harris L, Mason S, Stringer B, Day B, Wray N, Gronostajski R, Bailey T, Boyd A, Richards L
The Journal of Neuroscience 2014;34(8):2921-2930

---

MiR-153 targets the nuclear factor-1 family and protects against teratogenic effects of ethanol exposure in fetal neural stem cells

Tsai P, Bake S, Balaraman S, Rawlings J, Holgate R, Dubois D, Miranda R
Biology Open 2014;3(8):741-758

---

NFI Transcription Factors Interact with FOXA1 to Regulate Prostate-Specific Gene Expression

Grabowska M, Elliott A, DeGraff D, Anderson P, Anumanthan G, Yamashita H, Sun Q, Friedman D, Hachey D, Yu X, Sheehan J, Ahn J, Raj G, Piston D, Gronostajski R, Matusik R
Molecular Endocrinology 2014;28(6):949-964

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-NFIB (HPA003956, Lot 000053777) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.