MAB7119

antibody from Novus Biologicals

Targeting: SREBF2 bHLHd2, SREBP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7119

Provider

Novus Biologicals

Product name

Mouse Monoclonal SREBP2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human SREBP2 in ELISA and Western Blot.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human SREBP2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL) and 15 µg of nuclear extract (Nuc). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SREBP2 Monoclonal Antibody (Catalog # MAB7119) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for SREBP2 at approximately 125 kDa and 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

SREBP2 in Human Kidney. SREBP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human SREBP2 Monoclonal Antibody (Catalog # MAB7119) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.