PA5-88943

antibody from Invitrogen Antibodies

Targeting: SREBF2 bHLHd2, SREBP2

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88943

Provider

Invitrogen Antibodies

Product name

SREBP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: Raji, A-431, Jurkat, HeLa, HepG2, Mouse liver, Rat testis; Cellular Location: COPII-coated vesicle membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, Multi-pass membrane protein, Nucleus Immunogen sequence: MDDSGELGGL ETMETLTELG DELTLGDIDE MLQFVSNQVG EFPDLFSEQL CSSFPGSGGS GSSSGSSGSS SSSSNGRGSS SGAVDPSVQR SFTQVTLPSF SPSAASPQAP TLQVKVSPTS VPTTPRATPI LQPRPQPQPQ PQTQLQQQTV MITPTFSTTP QTRIIQQPLI YQNAATSFQV LQPQVQSLVT SSQVQPVTIQ QQVQTVQAQR VLTQTANGTL

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.16 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Hyperlipidemic hypersensitivity to lethal microbial inflammation and its reversal by selective targeting of nuclear transport shuttles.

Liu Y, Zienkiewicz J, Boyd KL, Smith TE, Xu ZQ, Hawiger J
Scientific reports 2021 Jun 7;11(1):11907

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SREBP2 in paraffin-embedded human liver cancer. Samples were incubated with SREBP2 Polyclonal antibody (Product # PA5-88943) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SREBP2 in paraffin-embedded mouse spinal cord. Samples were incubated with SREBP2 Polyclonal antibody (Product # PA5-88943) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Differential effect of pathway-selective NTM peptides in cultured cells. ( A-C ) Treatment with Imp alpha5-selective NTM, cSN50.1alpha inhibits nuclear import of SRTFs: NF-kappaB RelA ( A ), phosphorylated STAT1 ( B ), and component of AP-1 complex, cFos ( C ) in LPS-stimulated RAW cells. Cells were pretreated with 30 uM NTM peptides (biselective cSN50.1, and two monoselective cSN50.1alpha and cSN50.1beta) for 30 min then incubated with LPS (10 ng/mL) for 6 h. ( D ) Nuclear import of nSREBP2 is inhibited by treatment with Imp beta1-selective NTM, cSN50.1beta peptide, in sterol-depleted HEK 293T cells. Cells were rapidly depleted of sterols by 15 min incubation with hydroxypropyl-beta-cyclodextrin (HPCD) and treated with NTM peptides (as indicated) for 2 h. ( E ) Metabolic transcription factor (MTF) ChREBP is transported to the nucleus primarily by Imp alpha5-mediated nuclear import pathway. HepG2 cells were incubated in low glucose (5.5 mM) medium for 24 h. Subsequently, they were treated with high glucose (HG, 25 mM) supplemented with 100 nM insulin (In) to facilitate intracellular influx of glucose. 30 uM NTMs (as indicated) were added and cells were incubated for additional 24 h. ( A ) through ( E ) nuclear content of SRTFs and MTFs was determined by quantitative immunoblotting analysis using LI-COR Odyssey infrared imaging system. Data is presented as a mean +- SEM (n = 4 for D , E ; n = 6 for A , B , C ). Statistical significance between LPS-stimulated cells (co