Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP54043PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP54043PU-N, RRID:AB_11143873
- Product name
- anti SREBF2 / SREBP2 (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 402-431aa) of human SREBF2 / SREBP2
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in HepG2 cell line lysates (35ug/lane) using SREBF2 / SREBP2 antibody Cat.-No. AP54043PU-N. This demonstrates the SREBF2 antibody detected the SREBF2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis with HepG2 cells using SREBF2 / SREBP2 antibody Cat.-No. AP54043PU-N, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human skin carcinoma, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SREBF2 antibody for IHC; Clinical relevance has not been evaluated.