GTX105164

antibody from GeneTex

Targeting: HSPA1L HSP70-HOM, hum70t

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX105164

Provider

GeneTex

Proper citation

GeneTex Cat#GTX105164, RRID:AB_1950522

Product name

HSP70 1L antibody [C1C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ?g whole cell lysate)A: H1299B: HeLa S3 (GTX14654)C: HepG2 (GTX27900)D: MOLT4 (GTX27912)E: Raji (GTX27908)7.5% SDS PAGEGTX105164 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSP70 1L(GTX105164) antibody at 1:500 dilution.