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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-114017 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSP701L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: DEFDHKRKEL EQMCNPIITK LYQGGCTGPA CGTGYVPGRP ATGPTIEEVD For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Predicted homology: Cow: 86%; Dog: 86%; Goat: 86%; Horse: 86%; Human: 100%; Mouse: 86%; Pig: 86%; Rat: 86%
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Rat, Lamprey using a Polyclonal HSP701L Antibody (Product # PA5-114017). Sample Type: 1: Lamprey CNS (20 µg). 2: Rat Brain Lysate (20 µg). Primary Dilution: 1:1,000. Secondary Antibody: Goat anti-Rabbit HRP. Secondary Dilution: 1:4,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Human Jurkat using a Polyclonal HSP701L Antibody (Product # PA5-114017).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Human MDA-MB-435s using a Polyclonal HSP701L Antibody (Product # PA5-114017). Antibody Dilution: 1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HEK293 Whole Cell Lysate using a Polyclonal HSP701L Antibody (Product # PA5-114017). HSPA1L was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with PA5-114017 with 1:200 dilution. Western blot was performed using PA5-114017 at 1:1,000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: HSPA1L IP with PA5-114017 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Formalin Fixed Paraffin Embedded Human Testis using a HSP701L Polyclonal Antibody (Product # PA5-114017) diluted 1:200. Secondary Antibody: Donkey anti-Rabbit-Cy3 diluted 1:200. Magnification: 20X.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HEK293 Whole Cell Lysate using a Polyclonal HSP701L Antibody (Product # PA5-114017). HSPA1L was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with PA5-114017 with 1:200 dilution. Western blot was performed using PA5-114017 at 1:1,000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: HSPA1L IP with PA5-114017 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.
