PA5-28202

antibody from Invitrogen Antibodies

Targeting: HSPA1L HSP70-HOM, hum70t

Antibody data

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Validation data

Product number: PA5-28202 - Provider product page
Provider: Invitrogen Antibodies
Product name: HSP701L Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse testis, rat testis, U87-MG, SK-N-SH, IMR32, SK-N-AS.
Concentration: 1 mg/mL

HSPA1L protein structure - PA5-28202 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HSP701L Polyclonal Antibody (Product # PA5-28202). Sample (30 µg whole cell lysate). A: H1299. B: HeLa S3. C: HepG2. D: MOLT4. E: Raji. 7.5% SDS PAGE. HSP701L Polyclonal Antibody (Product # PA5-28202) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HSP701L was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HSP701L Polyclonal Antibody (Product # PA5-28202) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HSP701L was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HSP701L Polyclonal Antibody (Product # PA5-28202) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HSP701L Polyclonal Antibody (Product # PA5-28202). Sample (30 µg whole cell lysate). A: H1299. B: HeLa S3. C: HepG2. D: MOLT4. E: Raji. 7.5% SDS PAGE. HSP701L Polyclonal Antibody (Product # PA5-28202) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HSP701L was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HSP701L Polyclonal Antibody (Product # PA5-28202) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HSP701L was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HSP701L Polyclonal Antibody (Product # PA5-28202) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.