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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- R31714 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NUMB Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NUMB antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NUMB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Numb antibody and Lane 1: human U87; 2: mouse Neuro-2a lysate. Predicted/observed size ~70KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) human RT4, 4) rat C6 and 5) mouse NIH 3T3 cell lysate with NUMB antibody. Predicted molecular weight ~70 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with NUMB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with NUMB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with NUMB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUMB antibody.
