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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- R35011 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NUMB Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NUMB antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Immunocytochemistry/Flow cytometry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the NUMB antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat brain lysate with NUMB antibody at 0.01ug/ml. Predicted/observed molecular weight ~70/80kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized mouse NIH 3T3 cells with NUMB antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human A431 cells with NUMB antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate tissue with NUMB antibody at 5ug/ml (top) and no primary antibody (bottom). Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human A431 cells with NUMB antibody (blue) at 10ug/ml and naive goat Ig (black).
