H00008650-D01P

antibody from Abnova Corporation

Targeting: NUMB C14orf41

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008650-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008650-D01P, RRID:AB_1676949

Product name

NUMB purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human NUMB protein.

Antigen sequence

MPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPH
QSPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGS
AAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWA
ALENKSKQRTNPSPTNPFSSDLQKTFEIEL


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NUMB expression in transfected 293T cell line (H00008650-T06) by NUMB MaxPab polyclonal antibody.Lane 1: NUMB transfected lysate(14.50 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between NUMB and APP. HeLa cells were stained with anti-NUMB rabbit purified polyclonal 1:1200 and anti-APP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)