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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501613 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501613, RRID:AB_11126372
- Product name
- NUMB mouse monoclonal antibody, clone OTI6B2 (formerly 6B2)
- Antibody type
- Monoclonal
- Description
- NUMB mouse monoclonal antibody, clone OTI6B2 (formerly 6B2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NUMB
- Isotype
- IgG
- Antibody clone number
- OTI6B2
- Vial size
- 100 µl
- Concentration
- 0.96 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC216230 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUMB antibody(TA501613), and then analyzed by flow cytometry.
- Validation comment
- FC
