PA1-16831

antibody from Invitrogen Antibodies

Targeting: NUMB C14orf41

Antibody data

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Validation data

Product number: PA1-16831 - Provider product page
Provider: Invitrogen Antibodies
Product name: NUMB Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: The target sequence has 90% sequence homology with rat.
Reactivity: Human, Mouse, Chicken/Avian
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 3.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

NUMB protein structure - PA1-16831 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of Numb isoforms 1 and 2 in A431 whole cell lysate (20 µg) using 0.5 µg/mL (Product # PA1-16831). ECL detection: 30 seconds.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NUMB in 20 µg A431 whole cell lysate. Samples were incubated in NUMB polyclonal antibody (Product # PA1-16831 using a dilution of 0.5 µg/mL. ECL detection: 30 seconds.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NUMB in 20 µg A431 whole cell lysate. Samples were incubated in NUMB polyclonal antibody (Product # PA1-16831 using a dilution of 0.5 µg/mL. ECL detection: 30 seconds.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NUMB in 0.5 mg/mL A431 lysate. Samples were incubated in NUMB polyclonal antibody (Product # PA1-16831). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of NUMB was achieved by transfecting U-87 MG cells with NUMB specific siRNAs (Silencer® select Product # s16466, Product # s16465). Western blot analysis (Fig. a) was performed using whole cell extracts from the NUMB knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed using NUMB Polyclonal Antibody (Product # PA1-16831) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NUMB isoforms.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-NUMB Polyclonal Antibody (Product # PA1-16831) on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), PC-3 (Lane 2), DU 145 (Lane 3), A549 (Lane 4), SH-SY5Y (Lane 5), HeLa (Lane 6) and Mouse lung (Lane 7) and numb major isoform 65, 72 kDa was observed across cell lines except A549. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).