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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-109214 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZBTB48 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse spleen tissue lysate, HepG2, PC-3M, SH-SY5Y, human liver cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZBTB48 in mouse spleen tissue lysate. Samples were incubated with ZBTB48 polyclonal antibody (Product # PA5-109214), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZBTB48 in SH-SY5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ZBTB48 polyclonal antibody (Product # PA5-109214), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZBTB48 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ZBTB48 polyclonal antibody (Product # PA5-109214), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ZBTB48 in paraffin-embedded rat kidney tissue. Samples were incubated with ZBTB48 polyclonal antibody (Product # PA5-109214), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ZBTB48 in paraffin-embedded mouse kidney tissue. Samples were incubated with ZBTB48 polyclonal antibody (Product # PA5-109214), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ZBTB48 in paraffin-embedded human liver cancer tissue. Samples were incubated with ZBTB48 polyclonal antibody (Product # PA5-109214), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ZBTB48 in paraffin-embedded human kidney tissue. Samples were incubated with ZBTB48 polyclonal antibody (Product # PA5-109214), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ZBTB48 in HeLa cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with ZBTB48 polyclonal antibody (Product # PA5-109214) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.
