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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB3381-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Activin A beta A subunit Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant human Activin A in ELISAs. Also recognizes Activin A precursor. The amino acid sequences of mature beta A subunits from human, mouse and rat are identical. Therefore, this antibody will also detect the mature beta A subunit from mouse and rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 69403
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Activin A programs the differentiation of human TFH cells.
Low miR-143/miR-145 Cluster Levels Induce Activin A Overexpression in Oral Squamous Cell Carcinomas, Which Contributes to Poor Prognosis.
Adipose Stromal Cell Contact with Endothelial Cells Results in Loss of Complementary Vasculogenic Activity Mediated by Induction of Activin A.
Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB.
The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases.
Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal.
Requirement of Smad3 for mast cell growth.
N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma.
Locci M, Wu JE, Arumemi F, Mikulski Z, Dahlberg C, Miller AT, Crotty S
Nature immunology 2016 Aug;17(8):976-84
Nature immunology 2016 Aug;17(8):976-84
Low miR-143/miR-145 Cluster Levels Induce Activin A Overexpression in Oral Squamous Cell Carcinomas, Which Contributes to Poor Prognosis.
Bufalino A, Cervigne NK, de Oliveira CE, Fonseca FP, Rodrigues PC, Macedo CC, Sobral LM, Miguel MC, Lopes MA, Paes Leme AF, Lambert DW, Salo TA, Kowalski LP, Graner E, Coletta RD
PloS one 2015;10(8):e0136599
PloS one 2015;10(8):e0136599
Adipose Stromal Cell Contact with Endothelial Cells Results in Loss of Complementary Vasculogenic Activity Mediated by Induction of Activin A.
Merfeld-Clauss S, Lupov IP, Lu H, March KL, Traktuev DO
Stem cells (Dayton, Ohio) 2015 Oct;33(10):3039-51
Stem cells (Dayton, Ohio) 2015 Oct;33(10):3039-51
Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
Sierra-Filardi E, Puig-Kröger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabéu C, Vega MA, Corbí AL
Blood 2011 May 12;117(19):5092-101
Blood 2011 May 12;117(19):5092-101
Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB.
Alexander S, Watt F, Sawaji Y, Hermansson M, Saklatvala J
Arthritis and rheumatism 2007 Nov;56(11):3715-25
Arthritis and rheumatism 2007 Nov;56(11):3715-25
The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases.
Kupershmidt L, Amit T, Bar-Am O, Youdim MB, Blumenfeld Z
Journal of neurochemistry 2007 Nov;103(3):962-71
Journal of neurochemistry 2007 Nov;103(3):962-71
Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal.
Greber B, Lehrach H, Adjaye J
Stem cells (Dayton, Ohio) 2007 Feb;25(2):455-64
Stem cells (Dayton, Ohio) 2007 Feb;25(2):455-64
Requirement of Smad3 for mast cell growth.
Funaba M, Nakaya K, Ikeda T, Murakami M, Tsuchida K, Sugino H
Cellular immunology 2006 Mar;240(1):47-52
Cellular immunology 2006 Mar;240(1):47-52
N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma.
Yoshinaga K, Inoue H, Utsunomiya T, Sonoda H, Masuda T, Mimori K, Tanaka Y, Mori M
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Sep 1;10(17):5702-7
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Sep 1;10(17):5702-7
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Hemoglobin Expression Induced by Activin A and Neutralization by Human/ Mouse/Rat Activin A Antibody. Recombinant Human/Mouse/ Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381). The ND50 is typically 0.02-0.06 µg/mL.
