PA5-21939

antibody from Invitrogen Antibodies

Targeting: INHBA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21939

Provider

Invitrogen Antibodies

Product name

Activin A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A549, H1299, HCT-116. Predicted reactivity: Mouse (100%), Rat (100%), Cat (100%), Pig (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.66 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Senescence marker activin A is increased in human diabetic kidney disease: association with kidney function and potential implications for therapy.

Bian X, Griffin TP, Zhu X, Islam MN, Conley SM, Eirin A, Tang H, O'Shea PM, Palmer AK, McCoy RG, Herrmann SM, Mehta RA, Woollard JR, Rule AD, Kirkland JL, Tchkonia T, Textor SC, Griffin MD, Lerman LO, Hickson LJ
BMJ open diabetes research & care 2019;7(1):e000720

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Inhibin beta-A in paraformaldehyde-fixed HeLa cells using an Inhibin beta-A polyclonal antibody (Product # PA5-21939) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A (Product # PA5-21939) antibody at 1:200 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 In vitro models of DKD-related injury. HK-2 studies following incubation with high glucose (HG, 25 mmol/L), transforming growth factor-beta1 (TGF-beta1; 5 ng/mL) and albumin (ALB, 5 mg/mL). Time-dependent effect of HG+TGF-beta1 on the mRNA/gene expression of activin A, monocyte chemoattractant protein-1 (MCP-1; an inflammatory marker), type I collagen (mesenchymal marker) and E-cadherin (epithelial marker) in injured HK-2 cells (A) Immunofluorescent staining of HK-2 cells injured by HG+TGF-beta1 at 12 hours (h) compared with baseline (B) Time-dependent effect for activin A and p16 expression in injured HK-2 cells (C) Protein levels of activin A in the supernatant of HK-2 treated with HG+TGF-beta1 (D) and combinations of HG with or without ALB (E) at 24 hours. Values are mean+-SEM. a P