Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF338 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Activin A beta A subunit Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse and rat Activin A in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice.
Small molecule inhibition of the CBFβ/RUNX interaction decreases ovarian cancer growth and migration through alterations in genes related to epithelial-to-mesenchymal transition.
Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity.
Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats.
Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target.
Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2.
Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps.
Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.
Activin A as a mediator of NK-dendritic cell functional interactions.
An activin receptor IIA ligand trap corrects ineffective erythropoiesis in β-thalassemia.
Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
Fgf-2 overexpression increases excitability and seizure susceptibility but decreases seizure-induced cell loss.
The cysteine-rich domain protein KCP is a suppressor of transforming growth factor beta/activin signaling in renal epithelia.
Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair.
Activin A is a potent activator of renal interstitial fibroblasts.
hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation.
Jin Q, Qiao C, Li J, Xiao B, Li J, Xiao X
Skeletal muscle 2019 May 27;9(1):16
Skeletal muscle 2019 May 27;9(1):16
Small molecule inhibition of the CBFβ/RUNX interaction decreases ovarian cancer growth and migration through alterations in genes related to epithelial-to-mesenchymal transition.
Carlton AL, Illendula A, Gao Y, Llaneza DC, Boulton A, Shah A, Rajewski RA, Landen CN, Wotton D, Bushweller JH
Gynecologic oncology 2018 May;149(2):350-360
Gynecologic oncology 2018 May;149(2):350-360
Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity.
Merfeld-Clauss S, Lu H, Wu X, March KL, Traktuev DO
Journal of cellular and molecular medicine 2018 Jan;22(1):173-184
Journal of cellular and molecular medicine 2018 Jan;22(1):173-184
Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats.
Hu J, Wang X, Tang YH, Shan YG, Zou Q, Wang ZQ, Huang CX
Molecular medicine reports 2018 Apr;17(4):5074-5080
Molecular medicine reports 2018 Apr;17(4):5074-5080
Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target.
Staudacher JJ, Yazici C, Carroll T, Bauer J, Pang J, Krett N, Xia Y, Wilson A, Papachristou G, Dirmeier A, Kunst C, Whitcomb DC, Fantuzzi G, Jung B
Scientific reports 2017 Oct 6;7(1):12786
Scientific reports 2017 Oct 6;7(1):12786
Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2.
Wallner C, Jaurich H, Wagner JM, Becerikli M, Harati K, Dadras M, Lehnhardt M, Behr B
Scientific reports 2017 Aug 29;7(1):9878
Scientific reports 2017 Aug 29;7(1):9878
Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps.
Yang Y, Zhang N, Crombruggen KV, Lan F, Hu G, Hong S, Bachert C
PloS one 2015;10(6):e0128564
PloS one 2015;10(6):e0128564
Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.
Chen L, Zhang W, Liang HF, Zhou QF, Ding ZY, Yang HQ, Liu WB, Wu YH, Man Q, Zhang BX, Chen XP
Cell communication and signaling : CCS 2014 Mar 17;12:18
Cell communication and signaling : CCS 2014 Mar 17;12:18
Activin A as a mediator of NK-dendritic cell functional interactions.
Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):1241-8
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):1241-8
An activin receptor IIA ligand trap corrects ineffective erythropoiesis in β-thalassemia.
Dussiot M, Maciel TT, Fricot A, Chartier C, Negre O, Veiga J, Grapton D, Paubelle E, Payen E, Beuzard Y, Leboulch P, Ribeil JA, Arlet JB, Coté F, Courtois G, Ginzburg YZ, Daniel TO, Chopra R, Sung V, Hermine O, Moura IC
Nature medicine 2014 Apr;20(4):398-407
Nature medicine 2014 Apr;20(4):398-407
Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
Yaden BC, Wang YX, Wilson JM, Culver AE, Milner A, Datta-Mannan A, Shetler P, Croy JE, Dai G, Krishnan V
The American journal of pathology 2014 Apr;184(4):1152-66
The American journal of pathology 2014 Apr;184(4):1152-66
Fgf-2 overexpression increases excitability and seizure susceptibility but decreases seizure-induced cell loss.
Zucchini S, Buzzi A, Barbieri M, Rodi D, Paradiso B, Binaschi A, Coffin JD, Marzola A, Cifelli P, Belluzzi O, Simonato M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Dec 3;28(49):13112-24
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Dec 3;28(49):13112-24
The cysteine-rich domain protein KCP is a suppressor of transforming growth factor beta/activin signaling in renal epithelia.
Lin J, Patel SR, Wang M, Dressler GR
Molecular and cellular biology 2006 Jun;26(12):4577-85
Molecular and cellular biology 2006 Jun;26(12):4577-85
Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
Maeshima A, Vaughn DA, Choi Y, Nigam SK
Developmental biology 2006 Jul 15;295(2):473-85
Developmental biology 2006 Jul 15;295(2):473-85
Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair.
Barksby HE, Hui W, Wappler I, Peters HH, Milner JM, Richards CD, Cawston TE, Rowan AD
Arthritis and rheumatism 2006 Feb;54(2):540-50
Arthritis and rheumatism 2006 Feb;54(2):540-50
Activin A is a potent activator of renal interstitial fibroblasts.
Yamashita S, Maeshima A, Kojima I, Nojima Y
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):91-101
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):91-101
hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation.
Oren A, Toporik A, Biton S, Almogy N, Eshel D, Bernstein J, Savitsky K, Rotman G
Gene 2004 Apr 28;331:17-31
Gene 2004 Apr 28;331:17-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Activin A in SK-BR-3 and HeLa Human Cell Lines. Activin A was detected in immersion fixed SK-BR-3 human breast cancer cell line (left panel, positive stain) and HeLa human cervical epithelial carcinoma cell line (right panel, negative stain) using Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticuli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Activin A in Human Breast Cancer Tissue. Activin A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human/Mouse/Rat Activin A beta A Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Hemoglobin Expression Induced by Activin A and Neutralization by Human/Mouse/Rat Activin A Antibody. Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338). The ND50 is typically 2-6 µg/mL.