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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- MAB307-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Erythropoietin R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P19235- Isotype
- IgG
- Antibody clone number
- 38409
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissues.
JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway.
Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Patterson SD, Rossi JM, Paweletz KL, Fitzpatrick VD, Begley CG, Busse L, Elliott S, McCaffery I
PloS one 2015;10(3):e0122149
PloS one 2015;10(3):e0122149
JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway.
De Grandis M, Cambot M, Wautier MP, Cassinat B, Chomienne C, Colin Y, Wautier JL, Le Van Kim C, El Nemer W
Blood 2013 Jan 24;121(4):658-65
Blood 2013 Jan 24;121(4):658-65
Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Khankin EV, Mutter WP, Tamez H, Yuan HT, Karumanchi SA, Thadhani R
PloS one 2010 Feb 16;5(2):e9246
PloS one 2010 Feb 16;5(2):e9246
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
Journal of neuroscience methods 2007 Aug 15;164(1):50-8
Journal of neuroscience methods 2007 Aug 15;164(1):50-8
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
