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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF1390 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Erythropoietin R Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Erythropoietin R is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P14753- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Erythropoietin promotes survival of retinal ganglion cells in DBA/2J glaucoma mice.
Zhong L, Bradley J, Schubert W, Ahmed E, Adamis AP, Shima DT, Robinson GS, Ng YS
Investigative ophthalmology & visual science 2007 Mar;48(3):1212-8
Investigative ophthalmology & visual science 2007 Mar;48(3):1212-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Erythropoietin R in Mouse Bone Marrow Cells. Erythropoietin R was detected in immersion fixed mouse bone marrow cells using Goat Anti-Mouse Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1390) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
