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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MA5-23824 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EpoR Monoclonal Antibody (38409)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 38409
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Iron modulation of erythropoiesis is associated with Scribble-mediated control of the erythropoietin receptor.
Khalil S, Delehanty L, Grado S, Holy M, White Z 3rd, Freeman K, Kurita R, Nakamura Y, Bullock G, Goldfarb A
The Journal of experimental medicine 2018 Feb 5;215(2):661-679
The Journal of experimental medicine 2018 Feb 5;215(2):661-679
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EpoR in UT‚7 human acute myeloid leukemia cell line. Samples were incubated in EpoR monoclonal antibody (Product # MA5-23824) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody. A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of EpoR in TF-1 human erythroleukemic cell line. Samples were incubated in EpoR monoclonal antibody (Product # MA5-23824) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody.
