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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- BS-1424R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPOR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human K562 Cell lysate (30 µg), probed with EPOR Polyclonal Antibody, Unconjugated (bs-1424R) at 1:1000 overnight at 4°C. Followed by incubation with secondary antibody at 1:20000 for 90 min at 37C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human K562 Cell lysate (30 µg), probed with EPOR Polyclonal Antibody, Unconjugated (bs-1424R) at 1:300 overnight at 4°C. Followed by incubation with secondary antibody at 1:20000 for 90 min at 37C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-EPOR Polyclonal Antibody (bs-1424R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-EPOR Polyclonal Antibody (bs-1424R), Unconjugated at 1:100 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human Molt-4 Cells were fixed with 2% paraformaldehyde (10 min). The cells were washed twice with 1X PBS and then incubated in 1X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Followed by incubation with EPOR Polyclonal Antibody, FITC Conjugated (bs-1424R-FITC) at 1 µg/1x10^6 cells for 30 min on ice. Primary antibody staining (green) was compared to Isotype Control (orange) and unstained cells (blue).
