Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF-322-PB - Provider product page
- Provider
- R&D Systems
- Product name
- Human Erythropoietin R Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with rhEpo and rhTpo is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P19235
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Khankin EV, Mutter WP, Tamez H, Yuan HT, Karumanchi SA, Thadhani R
PloS one 2010 Feb 16;5(2):e9246
PloS one 2010 Feb 16;5(2):e9246
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
Journal of neuroscience methods 2007 Aug 15;164(1):50-8
Journal of neuroscience methods 2007 Aug 15;164(1):50-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Erythropoietin R at approximately 70-78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Erythropoietin R by Simple WesternTM. Simple Western lane view shows lysates of UT-7 human acute myeloid leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Erythropoietin R at approximately 76 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.