Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB6926 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-Erythropoietin R (Y426) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Phospho-Erythropoietin R (Y426) in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P19235
- Isotype
- IgG
- Antibody clone number
- 690710
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Erythropoietin R (Y426) by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line untreated (-) or treated (+) with 300 ng/mL Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody (Catalog # MAB6926) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 64 to 70 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (lower panel, Catalog # MAB3072). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Erythropoietin R (Y426) by Simple WesternTM. Simple Western lane view shows lysates of UT-7 human acute myeloid leukemia cell line untreated (-) or treated (+) with 300 ng/mL Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 70 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody (Catalog # MAB6926). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.