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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-55354 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- API5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: TVEELYRNYG ILADATEQVG QHKDAYQVIL DGVKGGTKEK RLAAQFIPKF FKHFPELADS AINAQLDLCE DEDVSIRRQA IKELPQFATG ENLPRVADIL TQLLQTDDSA EFNLVN Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of API5 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an API5 Polyclonal Antibody (Product # PA5-55354).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of API5 in human cell line U-2 OS using API5 Polyclonal Antibody (Product # PA5-55354). Staining shows localization to nuclear speckles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of API5 in human urinary bladder using API5 Polyclonal Antibody (Product # PA5-55354) shows strong nuclear positivity in urothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of API5 in human placenta using API5 Polyclonal Antibody (Product # PA5-55354) shows moderate nuclear positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of API5 in human skin using API5 Polyclonal Antibody (Product # PA5-55354) shows strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of API5 in human pancreas using API5 Polyclonal Antibody (Product # PA5-55354) shows moderate nuclear positivity in exocrine glandular cells.
