Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-46223 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46223, RRID:AB_10009455
- Product name
- Rabbit Polyclonal API5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: API5 Antibody [NBP1-46223] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. API5 was also immunoprecipitated by rabbit anti-API5 antibody NBP1-46224.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: API5 Antibody [NBP1-46223] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-API5 antibody A302-784A used for WB at 0.1 ug/ml. detection:Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: API5 Antibody [NBP1-46223] - Detection of human API5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-API5 antibody NBP1-46223 (lot NBP1-46223-2) used for IP at 3 ug per reaction. API5 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46223-1) and another rabbit anti-API5 antibody. For blotting immunoprecipitated API5, NBP1-46223 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: API5 Antibody [NBP1-46223] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-API5 used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: API5 Antibody [NBP1-46223] - Detection of human API5 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-API5 (NBP1-46223). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: API5 Antibody [NBP1-46223] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-API5 used at 1:200 (1 ug/ml) Detection: DAB