PA5-92964

antibody from Invitrogen Antibodies

Targeting: TUFM EF-TuMT, EFTu

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92964

Provider

Invitrogen Antibodies

Product name

TUFM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ADAVQDSEMV ELVELEIREL LTEFGYKGEE TPVIVGSALC ALEGRDPELG LKSVQKLLDA VDTYIPVPAR DLEKPFLLPV EAVYSVPGRG TVVTGTLERG ILKKGDECEL LGHSKNIRTV VTGIEMFHKS LERAEAGDNL GALVRGLKRE DLRRGLVMVK PGSIKPHQKV EAQVYILSKE EGGRHKPFVS HFMPVMFSLT WDMACRIILP PEKELAMPGE DLKFNLILRQ PMILEKGQRF TLRDGNRTIG TGLVTNTLAM TEEEKNIKWG; Positive Samples: HT-29, HepG2, HeLa, A-431, Jurkat, Mouse kidney, Mouse liver, Rat kidney, Rat liver; Cellular Location: Mitochondrion

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.31 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TUFM in extracts of various cell lines using TUFM Polyclonal Antibody (Product # PA5-92964) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MCF7 cells using TUFM Polyclonal antibody (Product # PA5-92964). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TUFM was performed in MCF7 cells using TUFM Polyclonal Antibody (Product # PA5-92964).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TUFM was performed in U2OS cells using TUFM Polyclonal Antibody (Product # PA5-92964).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TUFM in paraffin-embedded human esophageal cancer using TUFM Polyclonal Antibody (Product # PA5-92964) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of TUFM was performed in 200 µg extracts of 293T cells using TUFM Polyclonal Antibody (Product # PA5-92964). Western blot was performed from the immunoprecipitate using TUFM Polyclonal Antibody.