Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27511 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TUFM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, NIH-3T3.
- Concentration
- 0.3 mg/mL
Submitted references Mitochondrial Protease ClpP is a Target for the Anticancer Compounds ONC201 and Related Analogues.
Novel compound mutations in the mitochondrial translation elongation factor (TSFM) gene cause severe cardiomyopathy with myocardial fibro-adipose replacement.
Graves PR, Aponte-Collazo LJ, Fennell EMJ, Graves AC, Hale AE, Dicheva N, Herring LE, Gilbert TSK, East MP, McDonald IM, Lockett MR, Ashamalla H, Moorman NJ, Karanewsky DS, Iwanowicz EJ, Holmuhamedov E, Graves LM
ACS chemical biology 2019 May 17;14(5):1020-1029
ACS chemical biology 2019 May 17;14(5):1020-1029
Novel compound mutations in the mitochondrial translation elongation factor (TSFM) gene cause severe cardiomyopathy with myocardial fibro-adipose replacement.
Perli E, Pisano A, Glasgow RIC, Carbo M, Hardy SA, Falkous G, He L, Cerbelli B, Pignataro MG, Zacara E, Re F, Della Monica PL, Morea V, Bonnen PE, Taylor RW, d'Amati G, Giordano C
Scientific reports 2019 Mar 25;9(1):5108
Scientific reports 2019 Mar 25;9(1):5108
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUFM using 30 µg of A) 293T and B) H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TUFM polyclonal antibody (Product # PA5-27511) at a dilution of 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TUFM Polyclonal Antibody (Product # PA5-27511). Sample (30 µg of whole cell lysate). Lane A:NIH-3T3 10% SDS PAGE. TUFM Polyclonal Antibody (Product # PA5-27511) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TUFM Polyclonal Antibody (Product # PA5-27511). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM Polyclonal Antibody (Product # PA5-27511) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TUFM Polyclonal Antibody (Product # PA5-27511). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with TUFM Polyclonal Antibody (Product # PA5-27511) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TUFM in paraformaldehyde-fixed HeLa cells using a TUFM polyclonal antibody (Product # PA5-27511) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUFM Polyclonal Antibody detects TUFM protein at mitochondria on H441 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H441 xenograft. TUFM Polyclonal Antibody (Product # PA5-27511) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUFM Polyclonal Antibody detects TUFM protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. TUFM Polyclonal Antibody (Product # PA5-27511) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUFM Polyclonal Antibody immunoprecipitates TUFM protein in IP experiments. IP samples: HepG2 whole cell extract. A. 35 µg HepG2 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of TUFM protein by 4 µg TUFM Polyclonal Antibody (Product # PA5-27511). 10 % SDS-PAGE. The immunoprecipitated TUFM protein was detected by TUFM Polyclonal Antibody (Product # PA5-27511) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of ONC201 and TR-57 are prevented by ClpP knockdown inSUM159 cells. (a) Immunoblot of lysates from SUM159 cells transfectedwith a nontargeting siRNA (siCTRL) or siRNA targeting ClpP (siClpP)and treated with either 10 muM ONC201, 150 nM TR-57, or DMSOfor 24 h. Blots shown are representative of three biological replicates.(b) Total protein synthesis measured in SUM159 cells transfected withsiCTRL or siClpP after treatment with ONC201, TR-57, cycloheximide,or DMSO. (c) Cell viability plots for SUM159 cells transfected withsiCTRL (black circles) or siClpP (white squares) using Hoechst stainfor total cell count after 48-h treatment with ONC201 or TR-57. Graphsshown in (b) and (c) are representative of 2 biological replicates.Error bars represent standard error of the mean (SEM) from replicates.
