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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-27512 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TUFM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji. Predicted reactivity: Mouse (94%), Rat (95%), Rhesus Monkey (96%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses.
Schwessinger B, Bahar O, Thomas N, Holton N, Nekrasov V, Ruan D, Canlas PE, Daudi A, Petzold CJ, Singan VR, Kuo R, Chovatia M, Daum C, Heazlewood JL, Zipfel C, Ronald PC
PLoS pathogens 2015 Mar;11(3):e1004809
PLoS pathogens 2015 Mar;11(3):e1004809
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SNU16 xenograft, using TUFM (Product # PA5-27512) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUFM Polyclonal Antibody immunoprecipitates TUFM protein in IP experiments. IP samples: HepG2 whole cell extract. A. 35 µg HepG2 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of TUFM protein by 4 µg TUFM Polyclonal Antibody (Product # PA5-27512). 10 % SDS-PAGE. The immunoprecipitated TUFM protein was detected by TUFM Polyclonal Antibody (Product # PA5-27512) diluted at 1:1,000.
