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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00009263-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009263-M03, RRID:AB_608559
- Product name
- Mouse Monoclonal DRAK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. STK17A - serine/threonine kinase 17a (apoptosis-inducing)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRAK1 Antibody (4D12) [H00009263-M03] - Western blot analysis of STK17A over-expressed 293 cell line, cotransfected with STK17A Validated Chimera RNAi (H00009263-R01V) Lane 1 non-transfected control. Blot probed with H00009263-M03. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRAK1 Antibody (4D12) [H00009263-M03] - Analysis of STK17A expression in transfected 293T cell line by STK17A monoclonal antibody (M03), clone 4D12. Lane 1: STK17A transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRAK1 Antibody (4D12) [H00009263-M03] - Analysis of STK17A expression in HepG2 (Cat # L019V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DRAK1 Antibody (4D12) [H00009263-M03] - Detection limit for recombinant GST tagged STK17A is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DRAK1 Antibody (4D12) [H00009263-M03] - Analysis of monoclonal antibody to STK17A on formalin-fixed paraffin-embedded human colon cancer. Antibody concentration 1.2 ug/ml
