NB600-488

antibody from Novus Biologicals

Targeting: ZIC1 ZIC, ZNF201

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB600-488

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-488, RRID:AB_2241976

Product name

Rabbit Polyclonal ZIC1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.

Di Giovannantonio LG, Di Salvio M, Omodei D, Prakash N, Wurst W, Pierani A, Acampora D, Simeone A
Development (Cambridge, England) 2014 Jan;141(2):377-88

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MicroRNA-195 targets ADP-ribosylation factor-like protein 2 to induce apoptosis in human embryonic stem cell-derived neural progenitor cells.

Zhou Y, Jiang H, Gu J, Tang Y, Shen N, Jin Y
Cell death & disease 2013 Jun 27;4:e695

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Foxc1 is required by pericytes during fetal brain angiogenesis.

Siegenthaler JA, Choe Y, Patterson KP, Hsieh I, Li D, Jaminet SC, Daneman R, Kume T, Huang EJ, Pleasure SJ
Biology open 2013 Jul 15;2(7):647-59

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Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP response.

Lipchina I, Elkabetz Y, Hafner M, Sheridan R, Mihailovic A, Tuschl T, Sander C, Studer L, Betel D
Genes & development 2011 Oct 15;25(20):2173-86

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A neuronal migratory pathway crossing from diencephalon to telencephalon populates amygdala nuclei

Fernando García-Moreno, María Pedraza, Luca G Di Giovannantonio, Michela Di Salvio, Laura López-Mascaraque, Antonio Simeone, Juan A De Carlos
Nature Neuroscience 2010 May;13(6):680-689

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ZIC1 Antibody [NB600-488] - Western blot analysis of Zic1 in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the lanes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ZIC1 Antibody [NB600-488] - WB analysis of Zic1 in overexpression lysate.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ZIC1 Antibody [NB600-488] - IHC analysis of Zic1 in mouse kidney using DAB with hematoxylin counterstain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ZIC1 Antibody [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation and density in the cerebral vasculature. Zic1 (green), BrdU (red), and IB4 (blue) triple immunofluorescence of E14.5 PDGFrbeta-cre; Foxc1fl-fl mutant and PDGFrbeta-cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. Image collected and cropped by CiteAb from the following publication (http://bio.biologists.org/cgi/doi/10.1242/bio.20135009), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ZIC1 Antibody [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants. Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) and IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h and Foxc1l/l embryos. Image collected and cropped by CiteAb from the following publication (http://bio.biologists.org/cgi/doi/10.1242/bio.20135009), licensed under a CC-BY licence.