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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- HPA004099 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA004099, RRID:AB_1855353
- Product name
- Anti-PI4K2B
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human PI4K2B, Gene description: phosphatidylinositol 4-kinase type 2 beta, Alternative Gene Names: FLJ11105, PI4KIIB, PIK42B, Validated applications: WB, IHC, ICC, Uniprot ID: Q8TCG2, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references
Phosphatidylinositol 4,5‐bisphosphate controls Rab7 and
PLEKHM
1 membrane cycling during autophagosome–lysosome fusion
Baba T, Toth D, Sengupta N, Kim Y, Balla T
The EMBO Journal 2019;38(8)
The EMBO Journal 2019;38(8)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and PI4K2B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402666).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
- Sample type
- Human
