Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-40922 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PER3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: DATLFCEPWT LNMQPAPLTS EEFKHVGLTA AVLSAHTQKE EQNYVDKFRE
- Concentration
- 0.5 mg/mL
Submitted references Impact of the acidic environment on gene expression and functional parameters of tumors in vitro and in vivo.
Rauschner M, Lange L, Hüsing T, Reime S, Nolze A, Maschek M, Thews O, Riemann A
Journal of experimental & clinical cancer research : CR 2021 Jan 6;40(1):10
Journal of experimental & clinical cancer research : CR 2021 Jan 6;40(1):10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-PER3 / Period Circadian Protein 3 polyclonal antibody (Product # PA5-40922).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Change of protein levels of PER3, GLS2, CREM and TXNIP in AT1 prostate and Walker-256 mammary carcinoma cells after 24 h under acidotic (pH 6.6) and/or hypoxic (pO 2 = 1.5 mmHg) conditions. n = 4-12; (*) p < 0.05, (**) p < 0.01 vs. control; ( # ) p < 0.05, ( ## ) p < 0.01 AT1 vs. Walker-256 cells
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Protein levels of PER3, GLS2, CREM and TXNIP in AT1 prostate and Walker-256 mammary carcinoma tumors in vivo 24 h after ( a ) inducing metabolic acidosis by forcing glycolytic metabolism or ( b ) intratumoral injection of lactic acid. n = 4-18; (*) p < 0.05, (**) p < 0.01 vs. control; ( # ) p < 0.05, ( ## ) p < 0.01 AT1 vs. Walker-256 cells