A301-145A

antibody from Invitrogen Antibodies

Targeting: NCOR1 hCIT529I10, hN-CoR, KIAA1047, MGC104216, N-CoR, PPP1R109, TRAC1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-145A

Provider

Invitrogen Antibodies

Product name

NCoR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse NCoR by western blot. Samples:Whole cell lysate (50 µg) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibody (Product # A301-145A; lot A301-145A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NCoR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCoR antibody A301-145A (lot A301-145A-2) used for IP at 6 µg per reaction. NCoR was also immunoprecipitated by rabbit anti-NCoR antibody A301-146A. For blotting immunoprecipitated NCoR, A301-145A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse NCoR by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa and (50 µg) from mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibody A301-145A (lot A301-145A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody (Product # A301-145A) and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody (Product # A301-145A). ChIP-seq validation performed by Diogenode, Denville, NJ.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NCoR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCoR antibody (Product # A301-145A; lot A301-145A-3) used for IP at 6 µg per reaction. NCoR was also immunoprecipitated by a previous lot of this antibody (lot A301-145A-2) and rabbit anti-NCoR antibody (Product # A300-146A). For blotting immunoprecipitated NCoR (Product # A301-145A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.