- GTX23482 - GeneTex NCOR1 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescent analysis of NCoR (green) showing staining in the nucleus and cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR polyclonal antibody (Product # GTX23482) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ?C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescent analysis of NCoR (green) showing staining in the nucleus and cytoplasm of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR polyclonal antibody (GTX23482) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ?C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry analysis of NCoR showing positive staining in the nucleus of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NCoR polyclonal antibody (GTX23482) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry analysis of NCoR showing positive staining in the nucleus of paraffin-treated Mouse breast tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NCoR polyclonal antibody (GTX23482) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GTX23482