H00051295-M01

antibody from Abnova Corporation

Targeting: ECSIT SITPEC

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051295-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00051295-M01, RRID:AB_607026

Product name

ECSIT monoclonal antibody (M01), clone 1B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ECSIT.

Antigen sequence

TCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSL
VPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASF
LQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVER
DLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQE
CGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPM
LKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMF
GLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHI
VGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKC
VYYHILRADLLPPEEREVEETPEEWNLYYPMQLDL
EYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQAT
MAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSS
AGLEEPPLPEDHQEEDDNLQRQQQGQS


Isotype

IgG

Antibody clone number

1B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between SMURF1 and ECSIT. HeLa cells were stained with anti-SMURF1 rabbit purified polyclonal 1:1200 and anti-ECSIT mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)