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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-60183 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECSIT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: HMEPDLSARV TIYQVPLPKD STGAADPPQP HIVGIQSPDQ QAALARHNPA RPVFVEGPFS LWLRNKCVYY HILRADLLPP EEREVEETPE EWNLYYPM Highest antigen sequence identity to the following orthologs: Mouse - 84%, Rat - 85%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ECSIT in human cell lines PC-3 and HeLa using a ECSIT Polyclonal Antibody (Product # PA5-60183). Corresponding ECSIT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ECSIT in mouse cell line NIH-3T3 and rat cell line NBT-II using a ECSIT Polyclonal Antibody (Product # PA5-60183).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ECSIT in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using an ECSIT Polyclonal Antibody (Product # PA5-60183).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of ECSIT in human cell line U-251 MG using ECSIT Polyclonal Antibody (Product # PA5-60183). Staining shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ECSIT in human colon using ECSIT Polyclonal Antibody (Product # PA5-60183) shows strong cytoplasmic positivity in glandular cells.
