Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SMARCD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-30239 targets SMARCD1 in WB applications and shows reactivity with Human and mouse samples. The PA5-30239 immunogen is recombinant fragment corresponding to a region within amino acids 315 and 515 of Human SMARCD1.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCD1 using 30µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SMARCD1 polyclonal antibody (Product # PA5-30239) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCD1 using 30µg of HCT116 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SMARCD1 polyclonal antibody (Product # PA5-30239) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SMARCD1 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a SMARCD1 polyclonal antibody (Product # PA5-30239) diluted at 1:500. Blue: Hoechst 33342 staining.
