HPA004101

antibody from Atlas Antibodies

Targeting: SMARCD1 BAF60A, CRACD1, Rsc6p

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA004101

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA004101, RRID:AB_1080026

Product name

Anti-SMARCD1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human SMARCD1, Gene description: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1, Alternative Gene Names: BAF60A, CRACD1, Rsc6p, Validated applications: ChIP, ICC, IHC, WB, Uniprot ID: Q96GM5, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Myofiber Baf60c controls muscle regeneration by modulating Dkk3-mediated paracrine signaling

Xu J, Li X, Chen W, Zhang Z, Zhou Y, Gou Y, Lv C, Jin L, Qiu X, Ma S, Wu Q, Liu T, Mi L, Yang Z, Yu T, Pan X, Feng Y, Shan P, Meng Z
Journal of Experimental Medicine 2023;220(9)

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-SMARCD1 (HPA004101, Lot A105546) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.