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LearnMAB625-100
antibody from R&D Systems
Targeting: TNFRSF1A
CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB625-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF RI/TNFRSF1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human TNF RI/TNFRSF1A in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) TGF-alpha , hTGF-beta 1, porcine (p) TGF-beta 1, rhTGF-beta 1, pTGF-beta 1.2, pTGF-beta 2, recombinant chicken TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-beta , or rhTNF RII is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 16805
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes.
Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
IRF5 is required for late-phase TNF secretion by human dendritic cells.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors.
trans fatty acids and systemic inflammation in heart failure.
Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
Yeap WH, Wong KL, Shimasaki N, Teo EC, Quek JK, Yong HX, Diong CP, Bertoletti A, Linn YC, Wong SC
Scientific reports 2016 Sep 27;6:34310
Scientific reports 2016 Sep 27;6:34310
Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
Steeland S, Puimège L, Vandenbroucke RE, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
The Journal of biological chemistry 2015 Feb 13;290(7):4022-37
The Journal of biological chemistry 2015 Feb 13;290(7):4022-37
CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
Denoyer A, Godefroy D, Célérier I, Frugier J, Riancho L, Baudouin F, Rostène W, Baudouin C
Mucosal immunology 2012 Nov;5(6):702-11
Mucosal immunology 2012 Nov;5(6):702-11
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
IRF5 is required for late-phase TNF secretion by human dendritic cells.
Krausgruber T, Saliba D, Ryzhakov G, Lanfrancotti A, Blazek K, Udalova IA
Blood 2010 Jun 3;115(22):4421-30
Blood 2010 Jun 3;115(22):4421-30
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
Lee SY, Cherla RP, Tesh VL
Infection and immunity 2007 Mar;75(3):1291-302
Infection and immunity 2007 Mar;75(3):1291-302
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Emsley HC, Smith CJ, Gavin CM, Georgiou RF, Vail A, Barberan EM, Illingworth K, Scarth S, Wickramasinghe V, Hoadley ME, Rothwell NJ, Tyrrell PJ, Hopkins SJ
BMC neurology 2007 Feb 28;7:5
BMC neurology 2007 Feb 28;7:5
Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death.
Ricci-Vitiani L, Pedini F, Mollinari C, Condorelli G, Bonci D, Bez A, Colombo A, Parati E, Peschle C, De Maria R
The Journal of experimental medicine 2004 Nov 15;200(10):1257-66
The Journal of experimental medicine 2004 Nov 15;200(10):1257-66
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors.
Hawari FI, Rouhani FN, Cui X, Yu ZX, Buckley C, Kaler M, Levine SJ
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 3;101(5):1297-302
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 3;101(5):1297-302
trans fatty acids and systemic inflammation in heart failure.
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
Experimental cell research 2004 Aug 15;298(2):560-73
Experimental cell research 2004 Aug 15;298(2):560-73
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 15;169(10):5660-72
A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
Bharucha AE, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD
The American journal of gastroenterology 2000 Sep;95(9):2338-42
The American journal of gastroenterology 2000 Sep;95(9):2338-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF RI/TNFRSF1A Inhibition of TNF-alpha-induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1) inhibits Recombinant Human TNF-alpha (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF-alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
